FB2026_03 , released September 17, 2026
Recombinant Construct: P{twi-lacZ.2PE}
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General Information
Symbol
P{twi-lacZ.2PE}
FlyBase ID
FBtp0002613
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZtwi.2PE
Size
Expression Data
lacZ reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Ecol\lacZtwi.2PE
Transgenic product class
Component
Use(s)
Encoded product / tool
lacZ
Description

Two tandem copies of the 260bp twi proximal element (PE) (located between -440 and -180 relative to the twi promoter) drive expression of lacZ; generated by fusing a copy of the 260bp PE upstream of twi promoter sequences from -440 to +160bp fused in frame to the lacZ coding region.

(Jiang et al., 1992)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 5
organism | ventral
(Jiang and Levine, 1993)
Additional Information
Statement
Reference

Strong expression of EcollacZ spanningthe 12-14 ventral-most cells of the embryo.

(Jiang and Levine, 1993)

In embryos at cellular blastoderm, expressed at high levels in the ventralmost 12-14 cells, with sharp boundaries at lateral borders.

(Jiang and Levine, 1993)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{twi-lacZ.2PE}
(Jiang and Levine, 1993)
descendant(s)
Comments

construct_comment: One of a series of constructs characterizing 5\' regulatory regions of twi, in particular the dl binding sites. Additional related constructs listed under P{twi-lacZ.PEe}.

(Jiang and Levine, 1993)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{twi-lacZ.2PE}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (5)