A mutated twi proximal element (PE), into which two copies of an E box from the rho promoter (with core sequence CATATG) have been inserted just upstream of the TD4 and TD5 dl binding sites, followed by twi sequence from -180 to +160bp relative to the transcription start site, are fused upstream of lacZ.
Expressed in the ventral-most 20 cells of the embryo.
construct_comment: One of a series of constructs characterizing 5\' regulatory regions of twi, in particular the dl binding sites. Additional related constructs listed under P{twi-lacZ.P}.