As for bcdΔ14 i.e. a deletion of bases 4468-4517 (coordinates of FBrf0047926) in bcd 3' UTR, with addition of a 1.3kb Ecol\lacZ gene fragment to aid detection in in situ hybridizations.
The locailzation ofbcdΔ14.T:EcollacZR is severely defective compaered to wild type bcdmRNA localization. Faint signal is detected along the anterior margin of theoocyte and very low levels of transcript are detected in the early embryo.