FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{BRE-Ubx-lacZ}
Open Close
General Information
Symbol
P{BRE-Ubx-lacZ}
FlyBase ID
FBtp0004600
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZUbx.bx
Size
Expression Data
lacZ reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Ecol\lacZUbx.bx
Transgenic product class
Component
Use(s)
Regulatory region(s)
Ubx_BRE
Encoded product / tool
lacZ
Description

Expression of Ecol\lacZUbx.PQ is driven by 8.8kb bx cDNA fused upstream of Ubx basal promoter. lacZ expression is driven by Ubx promoter region and the bx region enhancer element, BRE.

(Qian et al., 1991)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 6
ectoderm of parasegment 6 -- parasegment 12
(Qian et al., 1991)
embryonic stage 10
parasegment 6 -- parasegment 12
(Qian et al., 1991)
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4
organism | 20-50% egg length

Comment: 25-50% egg length

(Qian et al., 1991)
embryonic stage 6
visceral trunk mesoderm of parasegment 6 -- parasegment 12
(Qian et al., 1991)
embryonic stage 13
parasegment 5 -- parasegment 12
(Qian et al., 1991)
Additional Information
Statement
Reference

The UbxFBtr0091350:pb-XREcollacZ reporterconstruct EcollacZUbx.bx drives expression in a pattern similar toendogenous Ubx expression. At the cellular blastoderm stage, it is expressedbetween 50% and 25% egg length in the posterior region of the embryo. At thebeginning of gastrulation, four stripes of expression begin to appear which areresovled into an pair-rule pattern in parasegments 6, 8, 10 and 12. Laterduring gastrulation the anterior border of expression is parasegemtn 5.Expression is detected in bothe the ectoderm and visceral mesoderm of theembryo, and later in development slight expression in the ventral neurogenicregion.

(Qian et al., 1991)
 
Marker for
Reflects expression of
Ubx
(Qian et al., 1993, Qian et al., 1991)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
8.8 kb BRE
(Qian et al., 1993)
8.8 kb BRE-Ubx-lacZ
(Qian et al., 1993)
P{BRE-Ubx-lacZ}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (5)