Construct: Contains an EcoRI-SalI Sgs3e fragment encoding upstream regulatory sequences (which also includes the Sgs7For coding region), and Sgs3e promoter sequence fused to the Adh coding region.
2.7kb of Sgs3 5' sequence (which includes the Sgs7 coding region) is fused at nucleotide +10 to the Adh transcribed region.
Contains Sgs3 sequence from -2.7kb relative to the start site to +10bp on the Sgs3 leader (which also contains the Sgs7 coding region), fused to the Adh coding region.