1.1kb fragment of Hsp83 promoter (containing the ATG codon, translation start site and NLS) drives the expression of a 0.8kb PstI ClaI P\T fragment from Ecol\lacZP\T.1911-(Δ2-3)-2183.hs (containing the exons but no IVS3 sequences) which in turn is fused in frame to a 3.4kb ClaI XhoI fragment carrying the 3' half of Ecol\lacZ fused to Tn\neoR (Friedrich and Soriano, 1991, Genes Dev 5: 1513-1523).