su(wa)suRS is identical to wild type su(wa) from the promoter region through to the codon encoding amino acid 963. It also carries the 3'-untranslated region and polyadenylation signal from the tra gene (corresponding to 957bp through to approximately 1260bp in the wild-type tra sequence) in place of the su(wa) 3'-untranslated segment (the segment from 5362bp through to 5857bp of the wild-type su(wa) is replaced).