FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{50/50-lacZ}
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General Information
Symbol
P{50/50-lacZ}
FlyBase ID
FBtp0006194
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZrho.50:50.kni
wrho.50:50.kni
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Ecol\lacZrho.50:50.kni
Transgenic product class
Component
Use(s)
Encoded product / tool
lacZ
Description

In vitro mutagenesis creates kni binding sites 50bp 5' and 3' of the dl1 and dl4 sites respectively.

(Arnosti et al., 1996)
Component allele wrho.50:50.kni
Transgenic product class
Component
Use(s)
Encoded product / tool
w
Description

Construct: w gene expression is governed by the same regulatory region, though in the opposite relative orientation, as Ecol\lacZrho.50:50.kni, i.e. 700bp rho promoter fragment, maps between -2.2 and -1.5kb upstream of the rho transcription start site, that lacks all four sna-binding sites and has one synthetic sna-binding site 50bp downstream of d4, with kni binding sites created by in vitro mutagenesis 50bp 5' and 3' of the dl1 and dl4 sites respectively.

(Arnosti et al., 1996)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
NEE-kni-lacZ
(Haecker et al., 2007)
NEE.kni
(Nibu et al., 1998)
P{50/50-lacZ}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (5)