Transgenic construct used in an analysis of Trithorax response elements (TREs) in the bxd region of Ubx. Contains two divergently transcribed reporters; Ecol\lacZUbx.P140 and a w minigene. Three fragments from the bxd/pbx region upstream of Ubx; an SspI-BamHI fragment (nucleotides 209,575 to 210,192), a HindIII-BglII fragment (nucleotides 214,871 to 221,674) and a BglII-EcoRI fragment (nucleotides 226,706 to 232, 519, all coordinates relative to GenBank accession number U31961) have been inserted in between the two divergent promoters.
Construct: Three fragments from the bxd/pbx region upstream of Ubx; an SspI-BamHI fragment (nucleotides 209,575 to 210,192), followed by a HindIII-BglII fragment (nucleotides 214,871 to 221,674), followed by a BglII-EcoRI fragment (nucleotides 226,706 to 232, 519, all coordinates relative to GenBank accession number U31961) are fused upstream of Ecol\lacZUbx.P140.