Transgenic construct used to study the mechanism of action of the gypsy insulator. Contains a reporter composed of the yolk protein fat body enhancer (FBE1) element upstream of the Yp2 promoter and 5' untranslated region fused to the lacZ coding region. Six copies of the third su(Hw) protein binding site of the gypsy insulator, with spacer DNA between su(Hw) protein binding sites 3 and 4 (such that there is 62bp between the centres of the two sites), have been inserted between the FBE1 element and the Yp2 promoter.