FB2026_02 , released June 18, 2026
Recombinant Construct: P{FRT+SceI(y+,w-)}
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General Information
Symbol
P{FRT+SceI(y+,w-)}
FlyBase ID
FBtp0011638
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

Transgene designed to assay the activity of the I-SceI endonuclease. Contains a non-functional 9kb HindIII w fragment (two-thirds of the first exon and 5kb of 3' untranslated region, modified to introduce a frameshift and three new restriction sites for EcoRI, NruI and XhoI) and the y+mDint2 marker. These sequences are flanked by a pair of RS(I-SceI) sites, which are in turn flanked by a pair of FRT sites.

Construct components
Component
Use(s)
Marker(s)
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{FRT+SceI(y+,w-)}
P{FRT-I-SceI-y+-Δ(w)::XEN-I-SceI-FRT}
Secondary FlyBase IDs
    References (2)