FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{FRT(GypEnOff)}
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General Information
Symbol
P{FRT(GypEnOff)}
FlyBase ID
FBtp0013525
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

Transgenic construct designed for use in an episomal enhancer blocking assay. Contains direct FRT sites flanking a non-functional rearranged w gene (the 3' portion is fused upstream of the promoter plus 5' portion), plus a v+t1.8 marker outside of the FRT sites. In the presence of FLP recombinase, the w sequence is excised onto an extrachromosomal episome and the normal 5'-3' organization is restored. The episomal w reporter contains a 670bp w eye enhancer upstream of the w promoter and coding sequence, with a gypsy\su(Hw)BR insulator inserted upstream of the w enhancer.

(Parnell and Geyer, 2000)
Construct components
Component
Use(s)
Also carries
FRT
Marker(s)
v+t1.8
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
eFRT-GypEnOff
(Parnell and Geyer, 2000)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P[GypEnOff]
(Parnell and Geyer, 2000)
P{FRT(GypEnOff)}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)