Transgenic construct that contains a Ecol\lacZarm.PV reporter flanked on each side by an insulator (scs' and scs respectively) to mitigate any chromosome position effects. A 3.7kb NotI fragment containing lncRNA:roX1 cDNA sequences has been inserted (upstream of the arm promoter of the lacZ reporter) to test whether the fragment has a role in dosage compensation. The lncRNA:roX1 fragment is in 5'-3' orientation relative to the lacZ coding region.
construct_comment: roX1 sequences present in this construct are not expected to be transcribed unless by chance the construct is inserted adjacent to a promoter in the genome.