Sense-antisense transcription (driven by two convergent arrays of UAS regulatory sequences) of a 1.4kb genomic fragment spanning exons 2-4 of w results in double stranded RNA (dsRNA) expression.
construct_comment: P{Sym-UAS-w} contains w sequences flanked on both sides by Scer\UAS regulatory sequences in opposite orientation (producing alleles wSym.Scer\UAS and wa.Sym.Scer\UAS). Expression from both of these Scer\UAS sequences should result in both sense and antisense copies of the w sequences, producing dsRNA which can cause RNAi (dsRNA interference).
construct_comment: P{Sym-UAS-w} contains w sequences flanked on both sides by Scer\UAS regulatory sequences in opposite orientation (producing alleles wSym.Scer\UAS and wa.Sym.Scer\UAS). Expression from both of these Scer\UAS sequences should result in both sense and antisense copies of the w sequences, producing dsRNA which can cause RNAi (dsRNA interference).