Sense-antisense transcription (driven by two convergent arrays of UAS regulatory sequences) of a 1.7kb fragment corresponding to nucleotides +619 to +2327 of Nop60B (coordinates according to AF097634) results in double stranded RNA (dsRNA) expression.
construct_comment: P{Sym-UAS-Nop60B} contains Nop60B sequences flanked on both sides by Scer\UAS regulatory sequences in opposite orientation (producing alleles Nop60BSym.Scer\UAS and Nop60Ba.Sym.Scer\UAS). Expression from both of these Scer\UAS sequences should result in both sense and antisense copies of the Nop60B sequences, producing dsRNA which can cause RNAi (dsRNA interference).