FB2026_03 , released September 17, 2026
Recombinant Construct: PBac{TRE-3xP3-EYFP,p-tTA-K10}
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General Information
Symbol
PBac{TRE-3xP3-EYFP,p-tTA-K10}
FlyBase ID
FBtp0017479
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

The PBac{TRE-3xP3-EYFP,p-tTA-K10} construct was designed for use as an enhancer construct, containing at its 3' end a potential enhancer trap sequence that consists of a minimal P\T promoter and first intron fused upstream of tTA driver sequence. The construct also contains the Avic\GFPEYFP.tetO.3xP3 allele as an internal reporter for tTA activity in insertion lines. However in a screen, insertions of PBac{TRE-3xP3-EYFP,p-tTA-K10} did not detect activity of endogenous enhancers.

(Horn et al., 2003)
Construct components
Component
Use(s)
Marker(s)
Avic\GFPEYFP.tetO.3xP3
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
PBac{TRE-3xP3-EYFP,p-tTA-K10}
(Horn et al., 2003, FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)