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General Information
Symbol
PBac{WHr}
FlyBase ID
FBtp0018842
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Tni\piggyBac
Construct components
Product class / Tool use(s)
Encoded product / tool
Marker(s)
Description

The PBac{WHr} transgene represents the reconstituted transgenic element generated in vivo when FLP is used to induce recombination between the FRT sites of two insertions of the PBac{WH} transgene. Like PBac{WH}, PBac{WHr} contains UAS regulatory sequences at its 5' end, oriented so that in the presence of a GAL4 driver, transcription from UAS is directed outwards from this end of the element. This can result in misexpression of neighbouring genomic loci when the element is inserted in the genome. In addition it contains a long (199bp) FRT site, a w+mC marker and a gypsy insulator sequence (at the 3' end of the element, downstream of the marker allele). PBac{WHr} is typically generated as part of a deletion-generation strategy; when the two insertions are in trans and are in the appropriate relative orientation, FLP-mediated recombination can result in deletion of the genomic region between the two original insertions along with production of the reconstituted PBac{WHr} element at the recombination point.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
descendant(s)
Comments
Stocks (15)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Secondary FlyBase IDs
    References (23)