The PBac{RB3.WH3} transgene represents a hybrid element produced by FLP-mediated recombination between an insertion of a PBac{RB} element and an insertion of a PBac{WH} element. PBac{RB3.WH3} consists of terminus derived from the Tni\piggyBac 3' end - a splice acceptor site - a single FRT site - w^+mC marker - terminus derived from the Tni\piggyBac 3' end. PBac{RB3.WH3} is typically generated as part of a deletion-generation strategy; when the two insertions are in trans and are in the appropriate relative orientation, FLP-mediated recombination can result in deletion of the genomic region between the two original insertions along with production of the hybrid element at the recombination point. FLP-mediated recombination between a PBac{RB} and a PBac{WH} insertion can result in either a PBac{RB5.WH5} or a PBac{RB3.WH3} hybrid element, depending on the relative genomic locations and orientations of the original insertions.