Transgenic construct used to study the gypsy insulator. Contains the entire y gene, including the wing and body enhancers. A gypsy\su(Hw)BR insulator sequence has been inserted between the y enhancers and the y coding region. The y sequence is followed by 1. a loxP cassette containing a gypsy\su(Hw)BR insulator sequence, and 2. the w+mC minigene.
construct_comment: Contains the entire y gene, including the wing and body enhancers. gypsy\su(Hw)BR430 is inserted at -893bp relative to the transcription start site, separating the y wing and body enhancers from the y promoter and coding region. A P1\loxP cassette containing gypsy\su(Hw)BR430 is inserted downstream of the y gene. w+mC is located downstream of the P1\loxP cassette.
Contains the entire y gene, including the wing and body enhancers. gypsy\su(Hw)BR430 is inserted at -893bp relative to the transcription start site, separating the y wing and body enhancers from the y promoter and coding region. A P1\loxP cassette containing gypsy\su(Hw)BR430 is inserted downstream of the y gene. w+mC is located downstream of the P1\loxP cassette.