Construct: Nucleotide substitution: G2457C. Nucleotide substitution: C2458T. Nucleotide substitution: C2459A. Nucleotide substitution: G2461A. Nucleotide substitution: G2462A. Nucleotide substitution: C2463G. Nucleotide substitution: G2464C. The Mad binding site 2451bp downstream of the ato transcriptional start site has been mutated.