As sphinx1 and sphinx2 share 86% nucleotide homology, both sphinx1 and sphinx2 are inactivated by P{UAS-sphinx.RNAi}.
UASt regulatory sequences drive expression of a 500bp cDNA fragment from the 5' end of the sphinx1 coding sequence, inserted as an inverted repeat (IR) in a modified pUAST vector, pUAST-R57, which includes a 282bp genomic fragment of Ret, containing introns 5 and 6 between the two IR fragments, to enhance the effect of RNAi.
UASt regulatory sequences drive expression of a 500bp cDNA fragment from the 5' end of the sphinx1 coding sequence, inserted as an inverted repeat (IR) in a modified pUAST vector, pUAST-R57, which includes a 282bp genomic fragment of Ret, containing introns 5 and 6 between the two IR fragments, to enhance the effect of RNAi.