FlyBase Recombinant Construct Report: P{UAS-λPVR.KD}

UAS regulatory sequences drive expression of a chimeric protein in which the Pvr extracellular ligand-binding domain has been replaced by the dimerization domain of the bacteriophage λ cI repressor (to induce constitutive dimerization of the encoded protein). A Tag:MYC epitope has been inserted at the junction of the λ and Pvr sequences. In addition, a N1134A amino acid replacement has been introduced into the Pvr intracellular domain (this results in a kinase-dead protein).

(Jekely et al., 2005)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Pvr^{KD.λ.UAS.Tag:MYC}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

(Jekely et al., 2005)

P{UAS-λPVR.KD}

(FlyBase, 1992-)

Secondary FlyBase IDs

References (3)

Report Sections

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