UASt regulatory sequences drive expression of an inverted repeat.
Possible off-target effects (low probability).
Expression under the control of Scer\GAL4pnr-MD237 results in lethality (either before or at the pupal stage), depending on the insertion line used.
Expression of P{GD10847} under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies.
Adults expressing P{GD10847} under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression of P{GD10847} under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver results in viable flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in early pupal lethality or late larval lethality.
Expression under the control of Scer\GAL4Mef2.PR results in split larval body wall muscles.
Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.
Possible off-target effects (low probability).
Expression under the control of Scer\GAL4pnr-MD237 results in lethality (either before or at the pupal stage), depending on the insertion line used.
Expression of P{GD10847} under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies.
Adults expressing P{GD10847} under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression of P{GD10847} under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver results in viable flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in early pupal lethality or late larval lethality.
Expression under the control of Scer\GAL4Mef2.PR results in split larval body wall muscles.
Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.