UASt regulatory sequences drive expression of sns, in which 14 candidate sites for phosphorylation of tyrosine have been mutated (amino acid replacements Y1128F, Y1133F, Y1152F, Y1162F, Y1174F, Y1186F, Y1196F, Y1233F, Y1247F, Y1248F, Y1255F, Y1258F, Y1263F and Y1474F). The coding sequence is tagged at the C-terminal end with a single Tag:HA tag.