P-element construct created in vivo: the hairpin-containing segment, carried in a pKC26-derived plasmid, was introduced into flies and inserted via site-specific recombination into a genomic copy of P{attP,y+,w3'}.
Both Scer\GAL4hh.PU- and Scer\GAL4en.PU-driven expression lead to off-target phenotypes in the wing - smaller wing posterior compartment and smaller wing cells -, which are not observed in the Setd3 null mutants, Setd3[KO]). vt180913.
P-element construct created in vivo: the hairpin-containing segment, carried in a pKC26-derived plasmid, was introduced into flies and inserted via site-specific recombination into a genomic copy of P{attP,y+,w3'}.
Both Scer\GAL4hh.PU- and Scer\GAL4en.PU-driven expression lead to off-target phenotypes in the wing - smaller wing posterior compartment and smaller wing cells -, which are not observed in the Setd3 null mutants, Setd3[KO]). vt180913.