FB2026_03 , released September 17, 2026
Recombinant Construct: P{FRT(Obp57d-e.KO.ScerSceI.RS)}
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General Information
Symbol
P{FRT(Obp57d-e.KO.Scer\SceI.RS)}
FlyBase ID
FBtp0051389
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

Donor construct for ends-out targeted replacement of the Obp57d and Obp57e loci. The construct contains flanking sequence from 5' of the Obp57d locus (including the CG13430 and Cpr57A genes), followed by a loxP cassette containing a w+* marker, followed by flanking sequence from 3' of the Obp57e locus (including the CG30148 gene). A RS(I-SceI) restriction site is present upstream of the 5' flanking sequence. The entire targeting region is contained within an FRT cassette. The construct is designed to precisely remove both the Obp57d and Obp57e genes, but to leave intact a putative poly-A additional signal (ensuring the termination of any residual transcription that might otherwise affect the expression of downstream genes). The loxP cassette containing the w+* marker can also be removed, leaving only 34bp of the loxP sequence.

(Matsuo et al., 2007)
Construct components
Component
Use(s)
Also carries
FRT
loxP
RS(I-SceI)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Obp57d/e targeting vector
(Matsuo et al., 2007)
P{FRT(Obp57d-e.KO.Scer\SceI.RS)}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)