FB2026_03 , released September 17, 2026
Recombinant Construct: M{MtnBgen*-gen*.EGFP}
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General Information
Symbol
M{MtnBgen*-gen*.EGFP}
FlyBase ID
FBtp0051701
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

Transgene designed to study DNA double-strand break repair by single strand annealing (SSA). Consists of MtnB promoter/enhancer sequence upstream of a direct repeat of a 259bp genomic fragment of the MtnB transcription unit (this repeat includes MtnB UTR and coding sequence) in which the two repeats are separated by a RS(I-SceI) site, and with EGFP fused downstream of the second repeat. A silent point mutation has been introduced into both of the direct repeats (so that they are identical). In this configuration, the EGFP protein is not expressed. EGFP protein is only expressed if a double-strand DNA break is induced at the RS(I-SceI) site by the I-SceI endonuclease, and the break is repaired by single strand annealing (SSA) of the flanking MtnB direct repeats.

(Kappeler et al., 2008)
Construct components
Component
Use(s)
Regulatory region(s)
MtnB
Tagged with
EGFP
Also carries
RS(I-SceI)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
MtnBgen*-gen*
(Kappeler et al., 2008)
M{MtnBgen*-gen*.EGFP}
(FlyBase, 1996-)
P{MtnBgen*-gen*.EGFP}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)