FlyBase Recombinant Construct Report: P{hid-EGFP.5F'E2FMut}

2.2kb fragment, containing the hid 5' E2F binding site carrying base pair substitutions that disrupt the binding of E2f1 proteins (mutated bases in lowercase: TggCtaGC), drives expression of EGFP tagged with a nuclear localization signal (Tag:NLS(SV40-largeT)).

(Tanaka-Matakatsu et al., 2009)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Component Alleles

Allele

Avic\GFP^{EGFP.hid.5'F-E2FMut.Tag:NLS(SV40-largeT)}

Reference(s)

(Garcia-Hughes et al., 2015, Bhaskar et al., 2014, Tanaka-Matakatsu et al., 2009)

Molecular data

(Tanaka-Matakatsu et al., 2009)

Phenotype Data

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P{W-EGFP.5F'E2FMut}

(FlyBase, 1992-)

P{hid-EGFP.5F'E2FMut}

(FlyBase, 1996-)

hid5'E2F-mut-GFP

(Bhaskar et al., 2014)

hid5'F-E2FMut

(Tanaka-Matakatsu et al., 2009)

hidmutE2F>GFP

(Garcia-Hughes et al., 2015)

Secondary FlyBase IDs

References (6)

Report Sections

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