2.2kb fragment, containing the hid 5' E2F binding site carrying base pair substitutions that disrupt the binding of E2f1 proteins (mutated bases in lowercase: TggCtaGC), drives expression of EGFP tagged with a nuclear localization signal (Tag:NLS(SV40-largeT)).
2.2kb fragment, containing the hid 5' E2F binding site carrying base pair substitutions that disrupt the binding of E2f1 proteins (mutated bases in lowercase: TggCtaGC), drives expression of EGFP tagged with a nuclear localization signal (Tag:NLS(SV40-largeT)).