FB2020_06, released Dec 22, 2020

Recombinant Construct: P{hid-EGFP.5F'E2FMut}

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General Information
Symbol
P{hid-EGFP.5F'E2FMut}
FlyBase ID
FBtp0053436
Feature type
transgenic_transposon
Component Allele(s)
Avic\GFPEGFP.hid.5'F-E2FMut.Tag:NLS(SV40-largeT)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
(Tanaka-Matakatsu et al., 2009)
Construct components
Component allele Avic\GFPEGFP.hid.5'F-E2FMut.Tag:NLS(SV40-largeT)
Product class / Tool use(s)
Encoded product / tool
EGFP
Tagged with
Tag:NLS(SV40-largeT)
Description

2.2kb fragment, containing the hid 5' E2F binding site carrying base pair substitutions that disrupt the binding of E2f1 proteins (mutated bases in lowercase: TggCtaGC), drives expression of EGFP tagged with a nuclear localization signal (Tag:NLS(SV40-largeT)).

(Tanaka-Matakatsu et al., 2009)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
molecular
tag
(Tanaka-Matakatsu et al., 2009)
reporter
gene
(Tanaka-Matakatsu et al., 2009)
Component Alleles
Allele
Avic\GFPEGFP.hid.5'F-E2FMut.Tag:NLS(SV40-largeT)
Reference(s)
(Garcia-Hughes et al., 2015, Bhaskar et al., 2014, Tanaka-Matakatsu et al., 2009)
Molecular data

2.2kb fragment, containing the hid 5' E2F binding site carrying base pair substitutions that disrupt the binding of E2f1 proteins (mutated bases in lowercase: TggCtaGC), drives expression of EGFP tagged with a nuclear localization signal (Tag:NLS(SV40-largeT)).

(Tanaka-Matakatsu et al., 2009)
Phenotype Data
Phenotypic ClassPhenotype Manifest InDetailed Description
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
P{W-EGFP.5F'E2FMut}
(FlyBase, 1992-)
P{hid-EGFP.5F'E2FMut}
(FlyBase, 1996-)
hid5'E2F-mut-GFP
(Bhaskar et al., 2014)
hid5'F-E2FMut
(Tanaka-Matakatsu et al., 2009)
hidmutE2F>GFP
(Garcia-Hughes et al., 2015)
Secondary FlyBase IDs
    References (6)