FB2026_03 , released September 17, 2026
Recombinant Construct: P{10XUAS-IVS-mCD8::GFP.TTS3}
Open Close
General Information
Symbol
P{10XUAS-IVS-mCD8::GFP.TTS3}
FlyBase ID
FBtp0056323
Feature type
transgenic_transposable_element
Component Allele(s)
Mmus\Cd8aIVS.10xUAS.GFP
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Description

The P{10XUAS-IVS-mCD8::GFP.TTS3} construct contains two copies of Mmus\Cd8aIVS.10xUAS.GFP in tail-to-tail orientation, separated by a 2.8kb gypsy-insulated spacer.

(Pfeiffer et al., 2010)
Construct components
Component allele Mmus\Cd8aIVS.10xUAS.GFP
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Mmus\Cd8a
Tagged with
GFP
Description

Ten copies of the UAS site followed by an Hsp70 basal promoter are fused upstream of a Mmus\Cd8a-Avic\GFP fusion protein. A 67bp intron derived from Mhc has been inserted in the 5' UTR. An SV40 transcriptional terminator is present.

(Pfeiffer et al., 2010)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

The P{10XUAS-IVS-mCD8::GFP.TTS3} construct contains two copies of Mmus\Cd8aIVS.10xScer\UAS.T:Avic\GFP in tail-to-tail orientation, separated by a 2.8kb gypsy-insulated spacer.

(Pfeiffer et al., 2010)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{10XUAS-IVS-mCD8::GFP.TTS3}
(FlyBase, 1996-)
pJFRC11
(Pfeiffer et al., 2010)
Secondary FlyBase IDs
    References (4)