The FRT cassette present in P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.H3.2.HA} has been excised (removing wild-type His3.3B sequences present within the cassette), allowing the mutated form of His3.3B (which contains the amino acid replacements S31A, A87S, I89V and G90M and which is also tagged at the C-terminal end with Tag:HA) to be expressed under the control of the His3.3B promoter.
The Scer\FRT cassette present in P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.H3.2.HA} has been excised (removing wild-type His3.3B sequences present within the cassette), allowing the mutated form of His3.3B (which contains the amino acid replacements S31A, A87S, I89V and G90M and which is also tagged at the C-terminal end with T:Ivir\HA1) to be expressed under the control of the His3.3B promoter (this mutated form of His3.3B is represented by His3.3BH3.2.T:Ivir\HA1).