The His3.3B promoter drives expression of the wild-type His3.3B coding sequence tagged at the C-terminal end with Tag:FLAG and followed by His3.3B 3' UTR sequences. The His3.3B coding sequence, Tag:FLAG tag and 3' UTR sequences are contained within an FRT cassette and can thus be removed by using FLP recombinase.
Promoterless wild-type form of His3.3B which is tagged at the C-terminal end with Tag:HA and followed by His3.3B 3' UTR sequences. The FRT cassette which is present in P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.WT.HA} prevents expression of this coding sequence.
The P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.WT.HA} construct consists of the His3.3B promoter, followed by an Scer\FRT cassette which contains the wild-type His3.3B coding sequence tagged at the C-terminal end with T:Zzzz\FLAG, His3.3B 3' UTR sequences and also contains a Avic\GFPE.Ubi marker. Following the Scer\FRT cassette is a promoterless wild-type form of His3.3B, which is tagged at the C-terminal end with T:Ivir\HA1 and followed by His3.3B 3' UTR sequences. When the Scer\FRT cassette is present, the wild-type His3.3B sequences in the cassette are expressed under the control of the His3.3B promoter (represented by His3.3BT:Zzzz\FLAG) and the wild-type promoterless His3.3B sequences following the cassette (represented by His3.3BScer\FRT.T:Ivir\HA1) are not expressed.