In the absence of a GAL4 driver, the P{UAS-DTH1} construct shows significant rescue of flies that lack ple function in the central nervous system. This is most likely due to expression driven by intragenic transcriptional regulatory elements.

UASt regulatory sequences are fused upstream of a genomic fragment that encodes the entire ple transcription unit, from which the first four introns and exons c and d (exons that are specific to the type 2, non-neural, isoform) have been deleted. This results in a transgene which expresses only the type 1 (neural) isoform.