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General Information
Symbol
PBac{IS.GAL4DBD.w-}
FlyBase ID
FBtp0065571
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

PBac{IS.GAL4DBD.w-} represents a transgenic construct generated in vivo using the InSITE system; an enhancer trap cassette that contains a minimal P\T promoter followed by sequence encoding a GAL4(DBD)::Zip- hemidriver, derived from the P{ID.GAL4DBD} donor element, has been inserted into a PBac{IT.GAL4.w-} target element, by first using FLP recombinase to release the donor cassette and then using phiC31:int integrase to insert this cassette into the attP site in the target element. Finally, the swap has been completed by using P1cre recombinase to remove the original GAL4 enhancer trap cassette present in the original PBac{IT.GAL4.w-} target element (this also removes a w+mC marker inserted during the second step, thus the final PBac{IS.GAL4DBD.w-} construct is not marked with w[+]). A single loxP site is present downstream of the GAL4(DBD)::Zip- hemidriver sequence.

Construct components
Product class / Tool use(s)
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
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Orientation
Symbol
Reference
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

A P1\loxP cassette containing w+mC and Scer\GAL4IT has been excised from PBac{IS.GAL4DBD.GAL4} to produce PBac{IS.GAL4DBD.w-}.

Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{IS.GAL4DBD.w-}
Secondary FlyBase IDs
    References (4)