P-element construct created in vivo: the hairpin-containing segment, carried in a pKC26-derived plasmid, was introduced into flies and inserted via site-specific recombination into a genomic copy of P{attP,y+,w3'}.
General comments :The amplicon is not represented as a sequence feature in FlyBase because it does not appear to overlap the former position of the targeted gene.
P-element construct created in vivo: the hairpin-containing segment, carried in a pKC26-derived plasmid, was introduced into flies and inserted via site-specific recombination into a genomic copy of P{attP,y+,w3'}.
Probable off-target effects.
Targeted gene annotation (CG40748) was removed in subsequent FlyBase update.
General comments :The amplicon is not represented as a sequence feature in FlyBase because it does not appear to overlap the former position of the targeted gene.