FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-mCD8-TN-XL-8aa}
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General Information
Symbol
P{UAS-mCD8-TN-XL-8aa}
FlyBase ID
FBtp0069945
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
UAS-mCD8-TN-XL-8aa
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPmCD8-TN-XL-8aa.UAS
Transgenic product class
Component
Use(s)
Encoded product / tool
Description

UAS regulatory sequences drive expression of the "TN-XL" protein to which the Mmus\Cd8a transmembrane domain has been added at the N-terminal end and a short Glycin-linker followed by six amino acids known to bind the PDZ domain of mint has been added at the C-terminal end. The aim was to produce a calcium ion sensor that would be targeted to axons when expressed using an appropriate GAL4 driver. However, axonal targeting of the protein is not seen when it is expressed in neurons of transgenic flies and the calcium sensitivity of the reporter is essentially lost. Instead, at low to medium expression levels, the protein highlights the somata efficiently. The protein seems to be trapped in the endoplasmic reticulum.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{UAS-TNNC2.XL.8aa}
P{UAS-mCD8-TN-XL-8aa}
Secondary FlyBase IDs
    References (6)