FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{W+G+R+.TS58A2xSce}
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General Information
Symbol
M{W+G+R+.TS58A2xSce}
FlyBase ID
FBtp0072937
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

phiC31:int-mediated recombination has been used to integrate the TS58A2xSce vector into the M{3xP3-RFP.attP'}ZH-58A landing site. The M{W+G+R+.TS58A2xSce} construct contains: Dmau\mariner 5'ITR - w+* marker - RS(I-SceI) site - 442bp of genomic sequence homologous to sequence flanking one side of the landing site - Avic\GFP3xP3.cUa marker - 1000bp of genomic sequence homologous to sequence flanking the other side of the landing site - RS(I-SceI) site - Disc\RFP3xP3.PB marker - Dmau\mariner 3'ITR. The RS(I-SceI) sites can each be used to induce a double stranded break which is repaired by the single-strand annealing pathway. Due to the configuration of the construct, in each case this leads to deletion of a transposable element end plus a marker gene, resulting in stabilisation of the transgene.

(Tkachuk et al., 2011)
Construct components
Component
Use(s)
Also carries
RS(I-SceI)
Marker(s)
Avic\GFP3xP3.cUa
Disc\RFP3xP3.PB
w+*
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
M{3xP3-RFP.attP}
(Tkachuk et al., 2011)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
M{W+G+R+.TS58A2xSce}
(FlyBase, 1996-)
W+(G+)R+
(Tkachuk et al., 2011)
Secondary FlyBase IDs
    References (2)