FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{3xP3-RFP.attP}
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General Information
Symbol
M{3xP3-RFP.attP}
FlyBase ID
FBtp0021844
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
3xP3-RFP
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

The M{3xP3-RFP.attP} element acts as a landing site for integration of DNA into the genome at a defined location; the element contains an attP site at its 5' end, which acts as a target site for phiC31:int-mediated integration of any attB site-containing donor plasmid. The element also contains a Disc\RFP3xP3.PB marker that is flanked by loxP sites (oriented in the same direction), allowing for excision of the marker using P1cre recombinase. The attP site present in this element contains a T-C transition at position 26 downstream of the TTG site. This mutation is outside of the attP 'core region' of 39bp (minimal attP length defined in PMID:10801973) and is also outside the 'footprint' region bound by phiC31:int integrase (PMID:11069650) and so is not expected to affect the function of the attP site.

Construct components
Component
Use(s)
Element type
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
(bases )
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (46)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Symbol Synonym
M{3XP3-RFP.attP}
M{3xP3-RFP.attP}
M{3xP3-RFPattP}
pM3xP3-RFPattP
pM{3xP3-RFPattP}
pmar(3)-3xP3-RFP-attP-mar(5)
Secondary FlyBase IDs
    References (12)