FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{UAST-lacZ.B}
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General Information
Symbol
M{UAST-lacZ.B}
FlyBase ID
FBtp0022220
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZUAS.cBa.Tag:NLS(SV40-largeT)
Size
Expression Data
Associated insertion(s)
3 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
Disc\RFP3xP3.PB
w+mC
Component allele Ecol\lacZUAS.cBa.Tag:NLS(SV40-largeT)
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
lacZ
Tagged with
Tag:NLS(SV40-largeT)
Description

UASt regulatory sequences drive expression of lacZ tagged with a nuclear localization signal (Tag:NLS(SV40-largeT)).

(Bischof et al., 2007)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

The resulting inserted construct consists of a P1\loxP site, followed by a Disc\RFP3xP3.PB marker, followed by another P1\loxP site, followed by a w+mC marker, followed by another P1\loxP site, followed by the Ecol\lacZT:SV40\nls2 marker. The presence of the P1\loxP sites mean that it is possible to eliminate the Disc\RFP3xP3.PB and w+mC markers by cre-mediated recombination.

(Bischof et al., 2007)

Generated by phiC31\int-mediated integration of pUAS-lacZattB into a target "landing site" insertion of the M{3xP3-RFP.attP} construct.

(Bischof et al., 2007)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
M{UAST-lacZ.B}
(FlyBase, 1996-)
UAS-lacZ
(Yu et al., 2013)
pUAST-lacZ-attB
(Yu et al., 2013)
Secondary FlyBase IDs
    References (5)