The following information accompanied stocks donated to the Bloomington Stock Center by Johannes Bischof and Konrad Basler, University of Zürich.
M{vas-int.Dm} carries a phiC31 integrase gene that has been engineered to have the codon bias of Drosophila melanogaster. Expression of the integrase is driven by the vasa promoter.  The construction of M{vas-int.Dm} is similar to the construction of M{vas-int.B} (FBtp0022219), which was described in Bischof et al., 2007 (PNAS 104: 3312--3317, FBrf0199320). Basically, the vasa promoter-phiC31 integrase sequence, marked with Avic\GFPE.3xP3 (FBal0103092) and flanked by attB sites, was integrated into M{3xP3-RFP.attP} via phiC31 integrase-mediated recombination.  Other than the altered integrase coding region, M{vas-int.Dm} is identical to M{vas-int.B}.
The presence both of Avic\GFPE.3xP3 and Disc\RFP3xP3.PB (FBal0193772) in M{vas-int.Dm} causes RFP and EGFP expression in late pupal and adult eyes. In practice, this leads to a pink eye color from the RFP gradually appearing in adult flies as they get older. This pink, however, is clearly distinct from the color resulting from expression of white.
M{vas-int.Dm}ZH-2A is a homozygous and hemizygous viable and fertile, X chromosome insertion at 2A.  It was isolated by phiC31 integrase-mediated recombination of the attB-vasa promoter-phiC31 integrase-attB sequence into M{3xP3-RFP.attP}ZH-2A.
M{vas-int.Dm}ZH-102D is a homozygous viable and fertile, fourth chromosome insertion at 102D.  It was isolated by phiC31 integrase-mediated recombination of the the attB-vasa promoter-phiC31 integrase-attB sequence into M{3xP3-RFP.attP}ZH-102D.
In Bischof et al, 2007, the following insertions from Supporting Information Table 4 are insertions of M{3xP3-RFP.attP}:
M{3xP3-RFP.attP}ZH-2A
M{3xP3-RFP.attP}ZH-51D
M{3xP3-RFP.attP}ZH-86Fb
M{3xP3-RFP.attP}ZH-102D
The following lines are insertions of M{3xP3-RFP.attP'}:
M{3xP3-RFP.attP'}ZH-22A
M{3xP3-RFP.attP'}ZH-51C
M{3xP3-RFP.attP'}ZH-58A
M{3xP3-RFP.attP'}ZH-68E
M{3xP3-RFP.attP'}ZH-86Fa
M{3xP3-RFP.attP'}ZH-96E
M{3xP3-RFP.attP} and M{3xP3-RFP.attP'} differ by only a few nucleotides.