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Bischof, J., Basler, K. (2007.11.7). Information on M{vas-int.Dm} stocks. 
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FBrf0200341
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To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: phiC31 constructs and insertions
Date: Wed, 07 Nov 2007  10:46:12  -0500
The following information accompanied stocks 
donated to the Bloomington Stock Center by 
Johannes Bischof and Konrad Basler, University of Z├╝rich.
M{vas-int.Dm} carries a phiC31 integrase gene 
that has been engineered to have the codon bias 
of Drosophila melanogaster. Expression of the 
integrase is driven by the vasa promoter.  The 
construction of M{vas-int.Dm} is similar to the 
construction of M{vas-int.B} (FBtp0022219), which 
was described in Bischof et al., 2007 (PNAS 104: 
3312--3317; 
http://flybase.net/reports/FBrf0199320.html). 
Basically, the vasa promoter-phiC31 integrase 
sequence, marked with Avic\GFP[E.3xP3] 
(FBal0103092) and flanked by attB sites, was 
integrated into M{3xP3-RFP.attP} via phiC31 
integrase-mediated recombination.  Other than the 
altered integrase coding region, M{vas-int.Dm} is identical to M{vas-int.B}.
The presence both of Avic\GFP[E.3xP3] and 
Disc\RFP[3xP3.PB] (FBal0193772) in M{vas-int.Dm} 
causes RFP and EGFP expression in late pupal and 
adult eyes. In practice, this leads to a pink eye 
color from the RFP gradually appearing in adult 
flies as they get older. This pink, however, is 
clearly distinct from the color resulting from expression of white.
M{vas-int.Dm}ZH-2A is a homozygous and hemizygous 
viable and fertile, X chromosome insertion at 
2A.  It was isolated by phiC31 integrase-mediated 
recombination of the attB-vasa promoter-phiC31 
integrase-attB sequence into M{3xP3-RFP.attP}ZH-2A.
M{vas-int.Dm}ZH-102D is a homozygous viable and 
fertile, fourth chromosome insertion at 102D.  It 
was isolated by phiC31 integrase-mediated 
recombination of the the attB-vasa 
promoter-phiC31 integrase-attB sequence into M{3xP3-RFP.attP}ZH-102D.
In Bischof et al, 2007, the following insertions 
from Supporting Information Table 4 are insertions of M{3xP3-RFP.attP}:
M{3xP3-RFP.attP}ZH-2A
M{3xP3-RFP.attP}ZH-51D
M{3xP3-RFP.attP}ZH-86Fb
M{3xP3-RFP.attP}ZH-102D
The following lines are insertions of M{3xP3-RFP.attP'}:
M{3xP3-RFP.attP'}ZH-22A
M{3xP3-RFP.attP'}ZH-51C
M{3xP3-RFP.attP'}ZH-58A
M{3xP3-RFP.attP'}ZH-68E
M{3xP3-RFP.attP'}ZH-86Fa
M{3xP3-RFP.attP'}ZH-96E
M{3xP3-RFP.attP} and M{3xP3-RFP.attP'} differ by only a few nucleotides.
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