FB2026_02 , released June 18, 2026
Recombinant Construct: Dmel\M{nanos-int.NLS}
Open Close
General Information
Symbol
M{nanos-int.NLS}
FlyBase ID
FBtp0022218
Feature type
transgenic_transposable_element
Component Allele(s)
phiC31\intnanos.Tag:NLS(SV40-largeT)
Size
Expression Data
Associated insertion(s)
3 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
Avic\GFPE.3xP3
Disc\RFP3xP3.PB
Component allele phiC31\intnanos.Tag:NLS(SV40-largeT)
Transgenic product class
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
phiC31:int
Tagged with
Tag:NLS(SV40-largeT)
Description

A nos promoter drives expression of phiC31:int integrase, which is tagged with a nuclear localization signal (Tag:NLS(SV40-largeT)). nos 3'UTR sequences are also present.

(Bischof et al., 2007)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

Generated by phiC31\int-mediated integration of p3xP3-EGFP.nos-int.NLS.attB into a target "landing site" insertion of the M{3xP3-RFP.attP} construct.

(Bischof et al., 2007)

The resulting inserted construct consists of a P1\loxP site, followed by a Disc\RFP3xP3.PB marker, followed by another P1\loxP site, followed by a Avic\GFPE.3xP3 marker, followed by another P1\loxP site, followed by phiC31\intnos.T:SV40\nls2, which provides the integrase source. The presence of the P1\loxP sites mean that it is possible to eliminate the Disc\RFP3xP3.PB and Avic\GFPE.3xP3 markers by cre-mediated recombination.

(Bischof et al., 2007)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
M{nanos-int.NLS}
(FlyBase, 1996-)
M{nos-int.NLS}
(FlyBase, 1996-)
nos-phiNLS
(Bischof et al., 2007)
Secondary FlyBase IDs
    References (5)