FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: PBac{3xHA-Phf7}
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General Information
Symbol
PBac{3xHA-Phf7}
FlyBase ID
FBtp0079804
Feature type
transgenic_transposable_element
Component Allele(s)
Phf73xN.Tag:HA
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Phf73xN.Tag:HA
Transgenic product class
genomic_DNA
(Yang et al., 2012)
wild_type
(Yang et al., 2012)
Component
Use(s)
Encoded product / tool
Phf7
Tagged with
Tag:HA
Description

Contains the BAC construct CH322-177L19, which includes Phf7. The Phf7 sequences are tagged at the N-terminal end with three copies of Tag:HA.

(Yang et al., 2012)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | male & spermatogenesis
male germline cyst
(Yang et al., 2012)
male germline stem cell
(Yang et al., 2012)
spermatogonium
(Yang et al., 2012)
Additional Information
Statement
Reference

Phf73xN.Tag:HA protein is expressed in male germline stem cells, male germline cysts, and spermatogonia; expression peaks in 4-8 cell spermatogonial cysts and declines sharply thereafter. HA-tagged Phf7 protein colocalizes with bam protein in the testis.

(Yang et al., 2012)
 
Marker for
Reflects expression of
Phf7
(Yang et al., 2012)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
HA(PHF7)
(Yang et al., 2012)
HA-phf7
(Smolko et al., 2020)
PBac{3XHA-PHF7}
(Shapiro-Kulnane et al., 2022, Smolko et al., 2020)
PBac{3xHA-Phf7}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (5)