UASt regulatory sequences drive expression of 'Minikarst', a dominant-negative form of kst. 'Minikarst' was built from a kst cDNA segment encoding amino acids 1-1605 and a genomic clone starting at amino acid 3200 through to the stop codon. It lacks spectrin repeats 14-28 but retains the actin-binding domain, the SH3 domain, the dimer nucleation site and the tetramerization site and encodes all spliceoforms in segment 33. The protein is tagged at the C-terminal end with mCherry fluorescent protein.
