Contains (from 5' to 3'): a subfragment of the HG4-1 hb cis-regulatory sequence present in Avic\GFPhb.HG4-1, a basal Hsp70 promoter, the hb P1 5' UTR including the first intron and the P2 promoter, the GFP coding sequence, the hb 3'UTR and an SV40 transcription termination sequence.
At embryonic stage 11 AvicGFPHG4-5-RNA labels embryonic ganglion mother cells. At embryonic stage 16 both AvicGFPHG4-5-RNA and -protein are detected in early- and late-born neurons of the embryonic CNS.