FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{EP-MS2}
Open Close
General Information
Symbol
P{EP-MS2}
FlyBase ID
FBtp0085357
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
24 available
Molecular map
Description and Uses
Description

The P{EP-MS2} construct is designed to introduce copies of the Tag:MS2 stem loop into the genome, such that this mRNA tag can be used to identify and study mRNAs with particular subcellular distributions. The construct contains a tagging cassette at its 3' end, consisting of UASp regulatory sequences that are directed towards the 3' end of the element followed by six copies of the Tag:MS2 stem loop. Expression can be driven from the UASp sequences via a GAL4 driver, resulting in transcription of any endogenous gene downstream of the insertion site, with the Tag:MS2 stem loops incorporated at the 5' end of this GAL4-induced mRNA. The subcellular localization of the tagged mRNA can be detected by introducing a second transgene or modified endogenous locus that encodes a fluorescent protein tagged with Tag:MCP (a protein that specifically binds to Tag:MS2).

(Jayanandanan et al., 2011)
Construct components
Component
Use(s)
Element type
Regulatory region(s)
UASp
Tagged with
Tag:MS2
Marker(s)
w+mC
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
P{EPg}
(Jayanandanan et al., 2011)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
EP-MS2
(Jayanandanan et al., 2011)
P{EP-MS2}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)