FB2026_03 , released September 17, 2026
Recombinant Construct: PBac{FRT.GFP}
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General Information
Symbol
PBac{FRT.GFP}
FlyBase ID
FBtp0087263
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

Contains the coding sequence of EGFP, but lacking a start codon. An FRT site is present upstream of the coding region. Recombination between the FRT sites present in each of the PBac{Ubi-p63E.FRT} and PBac{FRT.GFP} constructs reconstitutes a functional "Ubi-GFP" transgene (PBac{Ubi-p63E.FRT.GFP}), with expression of EGFP driven by the Ubi-p63E promoter. This system has been combined with a marker of cell recombination status (P{lacO.256x}60F) to produce a two-part labeling system called "syncytial tracing by recombination-induced transcription" (STRIT).

(McLean and Cooley, 2013)
Construct components
Component
Use(s)
Encoded product / tool
EGFP
Also carries
FRT
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
FRT.GFP
(McLean and Cooley, 2013)
PBac{FRT.GFP}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)