FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-shg-tension-sensor}
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General Information
Symbol
P{UAS-shg-tension-sensor}
FlyBase ID
FBtp0095463
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component allele shgtension.UAS
Transgenic product class
Component
Use(s)
Description

UAS regulatory sequences drive expression of shg coding sequences with a tension-sensing module inserted between the transmembrane domain and arm-binding site. The tension-sensing module comprises the mTFP1 and Venus fluorescent proteins separated by a nanospring protein domain from spider silk. In the relaxed state, the two fluorophores are close enough to allow FRET. The spider silk domain stretches in response to pico Newton forces, reducing FRET.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
E-cadherin tension sensor
P{UAS-shg-tension-sensor}
Ubi-cadmTFP
Secondary FlyBase IDs
    References (3)