Open Close
General Information
Symbol
P{UAS-shg-tension-sensor}
FlyBase ID
FBtp0095463
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
Construct components
Component allele shgtension.UAS
Product class / Tool use(s)
Description

UAS regulatory sequences drive expression of shg coding sequences with a tension-sensing module inserted between the transmembrane domain and arm-binding site. The tension-sensing module comprises the mTFP1 and Venus fluorescent proteins separated by a nanospring protein domain from spider silk. In the relaxed state, the two fluorophores are close enough to allow FRET. The spider silk domain stretches in response to pico Newton forces, reducing FRET.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
Component Alleles
Reference(s)
Molecular data

UAS regulatory sequences drive expression of shg coding sequences with a tension-sensing module inserted between the transmembrane domain and arm-binding site. The tension-sensing module comprises the mTFP1 and Venus fluorescent proteins separated by a nanospring protein domain from spider silk. In the relaxed state, the two fluorophores are close enough to allow FRET. The spider silk domain stretches in response to pico Newton forces, reducing FRET.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
E-cadherin tension sensor
P{UAS-shg-tension-sensor}
Ubi-cadmTFP
Secondary FlyBase IDs
    References (2)