UAS regulatory sequences are followed by the Mmus\Cd8a membrane domain and an FRT cassette containing the GFP coding region. The cassette is followed by the mRFP(Unk) coding region. In the absence of FLP recombinase a Mmus\Cd8a-GFP fusion protein is expressed under the control of the UAS regulatory sequences.
Scer\UAS regulatory sequences are followed by the Mmus\Cd8a membrane domain and an Scer\FRT cassette containing the Avic\GFP coding region. The cassette is followed by the Disc\RFP coding region. In the absence of FLPase, a Mmus\Cd8a-Avic\GFP fusion protein is expressed under the control of the Scer\UAS regulatory sequences.