FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{UAS-automiW}
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General Information
Symbol
M{UAS-automiW}
FlyBase ID
FBtp0108829
Feature type
transgenic_transposable_element
Component Allele(s)
wUAS.automiW
Also Known As
automiW
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele wUAS.automiW
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
w
Tagged with
GFP
Description

UAS regulatory sequences drive expression of a transcription unit that expresses two artificial miRNAs that target the w gene (miW-1 and miW-2). These miRNA sequences are embedded in an intron which is followed by an exon containing the coding sequence for GFP.

(Besnard-Guérin et al., 2015)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

M{UAS-automiW} acts as a biosensor for AGO2-mediated silencing. The miRNA sequences encoded by the wScer\UAS.automiW transcription unit in the construct are complementary to the w+mC marker present in the construct, thus if AGO2-mediated silencing is functional, expression of wScer\UAS.automiW in a w[-] background using a GAL4 driver results in flies with white eyes. If AGO2-mediated silencing is compromised, this silencing is inhibited, resulting in flies with pigmented eyes.

(Besnard-Guérin et al., 2015)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
M{UAS-automiW}
(FlyBase, 1996-)
M{UASp-automiW}
(FlyBase, 1992-)
UAS-miR(white), (w+)
(Besnard-Guérin et al., 2015)
automiW
(Angelova et al., 2020, Besnard-Guérin et al., 2015)
Secondary FlyBase IDs
    References (5)