Scer\UAS regulatory sequences drive expression of 'ssPA-GCaMP6f', a superfolder photoactivatable fluorescent Ca[2+] reporter. The reporter shows strong near-UV dependent photoactivation and large responses to Ca[2+]. It consists of the M13 fragment of T:Ggal\MLCK, a circularly permutated GFP (cpGFP) and the calcium binding domain of T:Rat\Cam. Several mutations which are necessary and sufficient for photoactivation and superfolding have been introduced into the cpGFP sequence. The short 'ssPA-GCaMP6f' variant lacks the MGS and RSET domains present at the N-terminal end of the 'sPA-GCaMP6f' variant.
Scer\UAS regulatory sequences drive expression of 'ssPA-GCaMP6f', a superfolder photoactivatable fluorescent Ca[2+] reporter. The reporter shows strong near-UV dependent photoactivation and large responses to Ca[2+]. It consists of the M13 fragment of T:Ggal\MLCK, a circularly permutated GFP (cpGFP) and the calcium binding domain of T:Rat\Cam. Several mutations which are necessary and sufficient for photoactivation and superfolding have been introduced into the cpGFP sequence. The short 'ssPA-GCaMP6f' variant lacks the MGS and RSET domains present at the N-terminal end of the 'sPA-GCaMP6f' variant.