FB2026_02 , released June 18, 2026
Recombinant Construct: P{hs-yB.Q486E.P501T}
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General Information
Symbol
P{hs-yB.Q486E.P501T}
FlyBase ID
FBtp0111301
Feature type
transgenic_transposable_element
Component Allele(s)
Zzzz\ZFNQ486E.P501T.hs.yB
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Zzzz\ZFNQ486E.P501T.hs.yB
Transgenic product class
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
Zzzz\ZFN
Description

P{hs-yB.Q486E.P501T} expresses the same zinc finger nuclease encoded by P{hs-yB} (FBtp0016057) except for substitution of sequences encoding Threonine for Proline at residue 501 and Glutamic acid for Glutamine for at residue 486. As above, the P501T substitution corrects a missense mutation to give the wild type FokI sequence. The Q486E substitution prevents homodimerization of the zinc finger nuclease. The construct was generated in a Casper-based vector.

(Trautman and Carroll, 2016.1.13)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
64271
w*; P{hs-yB.Q486E.P501T}2C/MKRS
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{hs-yB.Q486E.P501T}
(Trautman and Carroll, 2016.1.13, FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)